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ECOS™ 21 (BL21)

Product No.

FYE207-5VL

ECOS X.png

Specification & Description:

This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The mutated rne gene (rne131) encodes a truncated RNase E enzyme that lacks the ability to degrade mRNA, resulting in an increase in mRNA stability. The transformation efficiency of BL21 is usually low, so the 6 minutes/ heat shock cold plating protocol is recommended if high efficiency is desired.

> 2 x 107, 100 μl x 5 vials

Efficiency
> 2 x 107 cfu/μg
Recommended for:
• Recombinant protein expression
• Subcloning

Pack Size:

Price:

Inquiry

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Datasheet

Applications

Features:

• Lack of Lon and Omp proteases allows high-level protein expression and accumulation
• Easy gene induction

Applications:

• IPTG induced gene expression in E. coli
• Ideal for high level expression of non-toxic recombinant protein expression
using T7-based expression vectors
• Subcloning

Quality Control:

Store At:

- 70℃

ECOS™ 101 (DH5α)

Product No.

FYE607-10VL

ECOS X.png

Specification & Description:

DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 14 kb).

> 1 x 108, 100 μl x 10 vials

Efficiency
> 1 x 108 cfu/μg
Recommended for:
• Subcloning
• General cloning

Pack Size:

Price:

Inquiry

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Datasheet

Applications

Features:

Suitable for cloning with large plasmid and cDNA library construction, and also allow blue-white colony screening.

Applications:

• Cloning and subcloning
• Scale-up application
• Blue/white screening

Quality Control:

Store At:

- 70℃

ECOS™ 101 (DH5α)

Product No.

FYE607-80VL

ECOS X.png

Specification & Description:

DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 14 kb).

> 1 x 108, 100 μl x 80 vials

Efficiency
> 1 x 108 cfu/μg
Recommended for:
• Subcloning
• General cloning

Pack Size:

Price:

Inquiry

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Datasheet

Applications

Features:

Suitable for cloning with large plasmid and cDNA library construction, and also allow blue-white colony screening.

Applications:

• Cloning and subcloning
• Scale-up application
• Blue/white screening

Quality Control:

Store At:

- 70℃

ECOS™ 101 (DH5α)

Product No.

FYE608-10VL

ECOS X.png

Specification & Description:

DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 14 kb).

> 3 x 108, 100 μl x 10 vials

>3 x 108 cfu/μg
Recommended for:
• High efficiency cloning
• Cloning low abundance transcripts
• General cloning
• Gene library

Pack Size:

Price:

Inquiry

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Datasheet

Applications

Features:

Suitable for cloning with large plasmid and cDNA library construction, and also allow blue-white colony screening.

Applications:

• Cloning and subcloning
• Scale-up application
• Blue/white screening

Quality Control:

Store At:

- 70℃

ECOS™ 101 (DH5α)

Product No.

FYE608-80VL

ECOS X.png

Specification & Description:

DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 14 kb).

> 3 x 108, 100 μl x 80 vials

>3 x 108 cfu/μg
Recommended for:
• High efficiency cloning
• Cloning low abundance transcripts
• General cloning
• Gene library

Pack Size:

Price:

Inquiry

Get a Quote
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Datasheet

Applications

Features:

Suitable for cloning with large plasmid and cDNA library construction, and also allow blue-white colony screening.

Applications:

• Cloning and subcloning
• Scale-up application
• Blue/white screening

Quality Control:

Store At:

- 70℃

ECOS™ 101 (DH5α)

Product No.

FYE609-10VL

ECOS X.png

Specification & Description:

DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 14 kb).

> 1 x 109, 100 μl x 10 vials

> 1 x 109 cfu/μg
Recommended for:
• Super high efficiency cloning
• Cloning lowest abundance transcripts
• Gene library

Pack Size:

Price:

Inquiry

Get a Quote
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Submit Request

Datasheet

Applications

Features:

Suitable for cloning with large plasmid and cDNA library construction, and also allow blue-white colony screening.

Applications:

• Cloning and subcloning
• Scale-up application
• Blue/white screening

Quality Control:

Store At:

- 70℃

ECOS™ 101 (DH5α)

Product No.

FYE609-80VL

ECOS X.png

Specification & Description:

DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 14 kb).

> 1 x 109, 100 μl x 80 vials

> 1 x 109 cfu/μg
Recommended for:
• Super high efficiency cloning
• Cloning lowest abundance transcripts
• Gene library

Pack Size:

Price:

Inquiry

Get a Quote
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Submit Request

Datasheet

Applications

Features:

Suitable for cloning with large plasmid and cDNA library construction, and also allow blue-white colony screening.

Applications:

• Cloning and subcloning
• Scale-up application
• Blue/white screening

Quality Control:

Store At:

- 70℃

ECOS™ X (DH5α)

Product No.

FYE610-10VL

ECOS X.png

Specification & Description:

DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 20 kb).
After 5-year invention based on our ECOSTM technology, Yeastern Biotech is able to offer our customers a brand new competent cell product with similar transformation efficiency to that of electroporation. ECOSTM X can be utilized for applications which require very high transformation efficiency such as the construction of genomic libraries. The “X” as shown in the name, stands for extreme, meaning this competent cell was designed to exceed limitation and to set new limitation! The “X” also represents “10” in Roman numeral; our new product aims to reach 1010 in its efficiency.

> 5 x 109, 100 μl x 10 vials

> 5 x 109 cfu/ μg
Recommended for:
• Super high efficiency cloning
• Cloning lowest abundance cDNA
• Gene library

Pack Size:

Price:

Inquiry

Get a Quote
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Datasheet

Applications

Features:

• One step in one tube and finish in 6 minutes • High efficiency as electro-competent cells
• Requires no expensive equipment

Applications:

• Efficient transformation of products from routine TA cloning • Transformation of minute amounts of PCR products
• Construction of a more representative gene library

Quality Control:

Store At:

- 70℃

ECOS™ X (DH5α)

Product No.

FYE610-80VL

ECOS X.png

Specification & Description:

DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 20 kb).
After 5-year invention based on our ECOSTM technology, Yeastern Biotech is able to offer our customers a brand new competent cell product with similar transformation efficiency to that of electroporation. ECOSTM X can be utilized for applications which require very high transformation efficiency such as the construction of genomic libraries. The “X” as shown in the name, stands for extreme, meaning this competent cell was designed to exceed limitation and to set new limitation! The “X” also represents “10” in Roman numeral; our new product aims to reach 1010 in its efficiency.

> 5 x 109, 100 μl x 80 vials

> 5 x 109 cfu/ μg
Recommended for:
• Super high efficiency cloning
• Cloning lowest abundance cDNA
• Gene library

Pack Size:

Price:

Inquiry

Get a Quote
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Submit Request

Datasheet

Applications

Features:

• One step in one tube and finish in 6 minutes • High efficiency as electro-competent cells
• Requires no expensive equipment

Applications:

• Efficient transformation of products from routine TA cloning • Transformation of minute amounts of PCR products
• Construction of a more representative gene library

Quality Control:

Store At:

- 70℃

ECOS™ Blue (XL1-Blue)

Product No.

FYE107-10VL

ECOS X.png

Specification & Description:

XL1-Blue is the most popular strain for blue/white screening. It is also an excellent host strain for routine cloning application using plasmid or lambda vectors. XL1-Blue cells are endonuclease (endA) deficient, which greatly improve the quality of mini-prep DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned DNA by the EcoK endonuclease system. The lacIqZ∆M15 gene on the F ́ episome allows blue-white color screening.

> 5 x 107, 100 μl x 10 vials

Efficiency
> 5 x 107 cfu/μg
Recommended for:
• Routine cloning and subcloning
• Blue/white screening

Pack Size:

Price:

Inquiry

Get a Quote
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Datasheet

Applications

Features:

• A strain modified to be ideal for blue/white screening • Tetracycline resistant

Applications:

• Routine cloning application • Subcloning
• cDNA library
• Blue/white screening

Quality Control:

Store At:

- 70℃

ECOS™ Blue (XL1-Blue)

Product No.

FYE107-80VL

ECOS X.png

Specification & Description:

XL1-Blue is the most popular strain for blue/white screening. It is also an excellent host strain for routine cloning application using plasmid or lambda vectors. XL1-Blue cells are endonuclease (endA) deficient, which greatly improve the quality of mini-prep DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned DNA by the EcoK endonuclease system. The lacIqZ∆M15 gene on the F ́ episome allows blue-white color screening.

> 5 x 107, 100 μl x 80 vials

Efficiency
> 5 x 107 cfu/μg
Recommended for:
• Routine cloning and subcloning
• Blue/white screening

Pack Size:

Price:

Inquiry

Get a Quote
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Submit Request

Datasheet

Applications

Features:

• A strain modified to be ideal for blue/white screening • Tetracycline resistant

Applications:

• Routine cloning application • Subcloning
• cDNA library
• Blue/white screening

Quality Control:

Store At:

- 70℃

ECOS™ Blue (XL1-Blue)

Product No.

FYE108-10VL

ECOS X.png

Specification & Description:

XL1-Blue is the most popular strain for blue/white screening. It is also an excellent host strain for routine cloning application using plasmid or lambda vectors. XL1-Blue cells are endonuclease (endA) deficient, which greatly improve the quality of mini-prep DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned DNA by the EcoK endonuclease system. The lacIqZ∆M15 gene on the F ́ episome allows blue-white color screening.

> 2 x 108, 100 μl x 10 vials

> 2 x 109 cfu/μg
Recommended for:
• Routine cloning and subcloning
• Blue/white screening

Pack Size:

Price:

Inquiry

Get a Quote
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Submit Request

Datasheet

Applications

Features:

• A strain modified to be ideal for blue/white screening • Tetracycline resistant

Applications:

• Routine cloning application • Subcloning
• cDNA library
• Blue/white screening

Quality Control:

Store At:

- 70℃

ECOS™ Blue (XL1-Blue)

Product No.

FYE108-80VL

ECOS X.png

Specification & Description:

XL1-Blue is the most popular strain for blue/white screening. It is also an excellent host strain for routine cloning application using plasmid or lambda vectors. XL1-Blue cells are endonuclease (endA) deficient, which greatly improve the quality of mini-prep DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned DNA by the EcoK endonuclease system. The lacIqZ∆M15 gene on the F ́ episome allows blue-white color screening.

> 2 x 108, 100 μl x 80 vials

> 2 x 109 cfu/μg
Recommended for:
• Routine cloning and subcloning
• Blue/white screening

Pack Size:

Price:

Inquiry

Get a Quote
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Submit Request

Datasheet

Applications

Features:

• A strain modified to be ideal for blue/white screening • Tetracycline resistant

Applications:

• Routine cloning application • Subcloning
• cDNA library
• Blue/white screening

Quality Control:

Store At:

- 70℃

ECOS™ Blue (XL1-Blue)

Product No.

FYE109-10VL

ECOS X.png

Specification & Description:

XL1-Blue is the most popular strain for blue/white screening. It is also an excellent host strain for routine cloning application using plasmid or lambda vectors. XL1-Blue cells are endonuclease (endA) deficient, which greatly improve the quality of mini-prep DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned DNA by the EcoK endonuclease system. The lacIqZ∆M15 gene on the F ́ episome allows blue-white color screening.

> 5 x 108, 100 μl x 10 vials

> 5 x 108 cfu/μg
Recommended for:
• Routine cloning and subcloning
• Blue/white screening
• cDNA library

Pack Size:

Price:

Inquiry

Get a Quote
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Submit Request

Datasheet

Applications

Features:

• A strain modified to be ideal for blue/white screening • Tetracycline resistant

Applications:

• Routine cloning application • Subcloning
• cDNA library
• Blue/white screening

Quality Control:

Store At:

- 70℃

ECOS™ Blue (XL1-Blue)

Product No.

FYE109-80VL

ECOS X.png

Specification & Description:

XL1-Blue is the most popular strain for blue/white screening. It is also an excellent host strain for routine cloning application using plasmid or lambda vectors. XL1-Blue cells are endonuclease (endA) deficient, which greatly improve the quality of mini-prep DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned DNA by the EcoK endonuclease system. The lacIqZ∆M15 gene on the F ́ episome allows blue-white color screening.

> 5 x 108, 100 μl x 80 vials

> 5 x 108 cfu/μg
Recommended for:
• Routine cloning and subcloning
• Blue/white screening
• cDNA library

Pack Size:

Price:

Inquiry

Get a Quote
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Submit Request

Datasheet

Applications

Features:

• A strain modified to be ideal for blue/white screening • Tetracycline resistant

Applications:

• Routine cloning application • Subcloning
• cDNA library
• Blue/white screening

Quality Control:

Store At:

- 70℃

ECOS™ 21 (BL21)

Product No.

FYE207-5VL

ECOS X.png

Specification & Description:

This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The mutated rne gene (rne131) encodes a truncated RNase E enzyme that lacks the ability to degrade mRNA, resulting in an increase in mRNA stability. The transformation efficiency of BL21 is usually low, so the 6 minutes/ heat shock cold plating protocol is recommended if high efficiency is desired.

> 2 x 107, 100 μl x 5 vials

Efficiency
> 2 x 107 cfu/μg
Recommended for:
• Recombinant protein expression
• Subcloning

Pack Size:

Price:

Inquiry

Get a Quote
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Submit Request

Datasheet

Applications

Features:

• Lack of Lon and Omp proteases allows high-level protein expression and accumulation
• Easy gene induction

Applications:

• IPTG induced gene expression in E. coli
• Ideal for high level expression of non-toxic recombinant protein expression
using T7-based expression vectors
• Subcloning

Quality Control:

Store At:

- 70℃

ECOS™ 21 (BL21)

Product No.

FYE207-40VL

ECOS X.png

Specification & Description:

This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The mutated rne gene (rne131) encodes a truncated RNase E enzyme that lacks the ability to degrade mRNA, resulting in an increase in mRNA stability. The transformation efficiency of BL21 is usually low, so the 6 minutes/ heat shock cold plating protocol is recommended if high efficiency is desired.

> 2 x 107, 100 μl x 40 vials

Efficiency
> 2 x 107 cfu/μg
Recommended for:
• Recombinant protein expression
• Subcloning

Pack Size:

Price:

Inquiry

Get a Quote
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Submit Request

Datasheet

Applications

Features:

• Lack of Lon and Omp proteases allows high-level protein expression and accumulation
• Easy gene induction

Applications:

• IPTG induced gene expression in E. coli
• Ideal for high level expression of non-toxic recombinant protein expression
using T7-based expression vectors
• Subcloning

Quality Control:

Store At:

- 70℃

ECOS™ 9-5 (JM109)

Product No.

FYE707-10VL

ECOS X.png

Specification & Description:

JM109, a K strain bacterium, carries mutations in recA and endA, leading to minimal recombination and improved quality of isolated plasmid DNA. In addition, the cells carry the F’ episome which allows blue/white screening for recombinant DNA. Thus, it has been a popular strain for routine subcloning. The cells can also be utilized for single-stranded DNA rescue when M13 or phagemid systems are used simultaneously. The JM109 strain is sensitive to all common antibiotics.
This product can become a powerful cloning tool when it is used in combination with Yeastern’s rapid ligation kit (#FYC003-100R). The ligation can be finished within 5 minutes and followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Try this strain when you are in urgent.

> 5 x 107, 100 μl x 10 vials

> 5 x 107 cfu/μg
Recommended for:
• Subcloning
• General cloning

Pack Size:

Price:

Inquiry

Get a Quote
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Datasheet

Applications

Features:

• Rapid growing strain
• Carries F' episome

Applications:

• Preparation of ssDNA
• Construction of gene library • Subcloning

Quality Control:

Store At:

- 70℃

ECOS™ 9-5 (JM109)

Product No.

FYE707-80VL

ECOS X.png

Specification & Description:

JM109, a K strain bacterium, carries mutations in recA and endA, leading to minimal recombination and improved quality of isolated plasmid DNA. In addition, the cells carry the F’ episome which allows blue/white screening for recombinant DNA. Thus, it has been a popular strain for routine subcloning. The cells can also be utilized for single-stranded DNA rescue when M13 or phagemid systems are used simultaneously. The JM109 strain is sensitive to all common antibiotics.
This product can become a powerful cloning tool when it is used in combination with Yeastern’s rapid ligation kit (#FYC003-100R). The ligation can be finished within 5 minutes and followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Try this strain when you are in urgent.

> 5 x 107, 100 μl x 80 vials

> 5 x 107 cfu/μg
Recommended for:
• Subcloning
• General cloning

Pack Size:

Price:

Inquiry

Get a Quote
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Datasheet

Applications

Features:

• Rapid growing strain
• Carries F' episome

Applications:

• Preparation of ssDNA
• Construction of gene library • Subcloning

Quality Control:

Store At:

- 70℃

ECOS™ 9-5 (JM109)

Product No.

FYE708-10VL

ECOS X.png

Specification & Description:

JM109, a K strain bacterium, carries mutations in recA and endA, leading to minimal recombination and improved quality of isolated plasmid DNA. In addition, the cells carry the F’ episome which allows blue/white screening for recombinant DNA. Thus, it has been a popular strain for routine subcloning. The cells can also be utilized for single-stranded DNA rescue when M13 or phagemid systems are used simultaneously. The JM109 strain is sensitive to all common antibiotics.
This product can become a powerful cloning tool when it is used in combination with Yeastern’s rapid ligation kit (#FYC003-100R). The ligation can be finished within 5 minutes and followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Try this strain when you are in urgent.

> 1 x 108, 100 μl x 10 vials

> 1 x 108 cfu/μg
Recommended for:
• High efficiency cloning
• Cloning low abundance transcripts
• General cloning
• Gene library

Pack Size:

Price:

Inquiry

Get a Quote
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Submit Request

Datasheet

Applications

Features:

• Rapid growing strain
• Carries F' episome

Applications:

• Preparation of ssDNA
• Construction of gene library • Subcloning

Quality Control:

Store At:

- 70℃

ECOS™ 9-5 (JM109)

Product No.

FYE708-80VL

ECOS X.png

Specification & Description:

JM109, a K strain bacterium, carries mutations in recA and endA, leading to minimal recombination and improved quality of isolated plasmid DNA. In addition, the cells carry the F’ episome which allows blue/white screening for recombinant DNA. Thus, it has been a popular strain for routine subcloning. The cells can also be utilized for single-stranded DNA rescue when M13 or phagemid systems are used simultaneously. The JM109 strain is sensitive to all common antibiotics.
This product can become a powerful cloning tool when it is used in combination with Yeastern’s rapid ligation kit (#FYC003-100R). The ligation can be finished within 5 minutes and followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Try this strain when you are in urgent.

> 1 x 108, 100 μl x 80 vials

> 1 x 108 cfu/μg
Recommended for:
• High efficiency cloning
• Cloning low abundance transcripts
• General cloning
• Gene library

Pack Size:

Price:

Inquiry

Get a Quote
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Submit Request

Datasheet

Applications

Features:

• Rapid growing strain
• Carries F' episome

Applications:

• Preparation of ssDNA
• Construction of gene library • Subcloning

Quality Control:

Store At:

- 70℃

ECOS™ 9-5 (JM109)

Product No.

FYE709-10VL

ECOS X.png

Specification & Description:

JM109, a K strain bacterium, carries mutations in recA and endA, leading to minimal recombination and improved quality of isolated plasmid DNA. In addition, the cells carry the F’ episome which allows blue/white screening for recombinant DNA. Thus, it has been a popular strain for routine subcloning. The cells can also be utilized for single-stranded DNA rescue when M13 or phagemid systems are used simultaneously. The JM109 strain is sensitive to all common antibiotics.
This product can become a powerful cloning tool when it is used in combination with Yeastern’s rapid ligation kit (#FYC003-100R). The ligation can be finished within 5 minutes and followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Try this strain when you are in urgent.

> 5 x 108, 100 μl x 10 vials

Recommended for:
• Subcloning
• General cloning

Pack Size:

Price:

Inquiry

Get a Quote
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Datasheet

Applications

Features:

• Rapid growing strain
• Carries F' episome

Applications:

• Preparation of ssDNA
• Construction of gene library • Subcloning

Quality Control:

Store At:

- 70℃

ECOS™ 9-5 (JM109)

Product No.

FYE709-80VL

ECOS X.png

Specification & Description:

JM109, a K strain bacterium, carries mutations in recA and endA, leading to minimal recombination and improved quality of isolated plasmid DNA. In addition, the cells carry the F’ episome which allows blue/white screening for recombinant DNA. Thus, it has been a popular strain for routine subcloning. The cells can also be utilized for single-stranded DNA rescue when M13 or phagemid systems are used simultaneously. The JM109 strain is sensitive to all common antibiotics.
This product can become a powerful cloning tool when it is used in combination with Yeastern’s rapid ligation kit (#FYC003-100R). The ligation can be finished within 5 minutes and followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Try this strain when you are in urgent.

> 5 x 108, 100 μl x 80 vials

• Subcloning
• General cloning

Pack Size:

Price:

Inquiry

Get a Quote
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Submit Request

Datasheet

Applications

Features:

• Rapid growing strain
• Carries F' episome

Applications:

• Preparation of ssDNA
• Construction of gene library • Subcloning

Quality Control:

Store At:

- 70℃

ECOS™ 10B (DH10B)

Product No.

FYE507-10VL

ECOS X.png

Specification & Description:

DH10B is an MC1061 derivative. This strain was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids.
While DH10B has been classically reported to be galU galK, the preliminary genome sequence for DH10B indicates that DH10B is actually galE galK galU+. Genome sequence indicates that DH10B is actually deoR+. Complete genome sequence has been published.

> 5 x 107, 100 μl x 10 vials

> 5 x 107 cfu/μg
Recommended for:
• Subcloning
• Genomic DNA cloning

Pack Size:

Price:

Inquiry

Get a Quote
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Datasheet

Applications

Features:

• Streptomycin resistant
• High transformation efficiency

Applications:

• Suitable for genomic DNA cloning
• Allow large inserts
• Cloning of methylated cytosine or adenine containing DNA
• Blue/white screening

Quality Control:

Store At:

- 70℃

ECOS™ 10B (DH10B)

Product No.

FYE507-80VL

ECOS X.png

Specification & Description:

DH10B is an MC1061 derivative. This strain was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids.
While DH10B has been classically reported to be galU galK, the preliminary genome sequence for DH10B indicates that DH10B is actually galE galK galU+. Genome sequence indicates that DH10B is actually deoR+. Complete genome sequence has been published.

> 5 x 107, 100 μl x 80 vials

> 5 x 107 cfu/μg
Recommended for:
• Subcloning
• Genomic DNA cloning

Pack Size:

Price:

Get a Quote
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Submit Request

Datasheet

Applications

Features:

• Streptomycin resistant
• High transformation efficiency

Applications:

• Suitable for genomic DNA cloning
• Allow large inserts
• Cloning of methylated cytosine or adenine containing DNA
• Blue/white screening

Quality Control:

Store At:

- 70℃

ECOS™ 10B (DH10B)

Product No.

FYE508-10VL

ECOS X.png

Specification & Description:

DH10B is an MC1061 derivative. This strain was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids.
While DH10B has been classically reported to be galU galK, the preliminary genome sequence for DH10B indicates that DH10B is actually galE galK galU+. Genome sequence indicates that DH10B is actually deoR+. Complete genome sequence has been published.

> 2 x 108, 100 μl x 10 vials

> 2 x 108 cfu/μg
Recommended for:
• Cloning of large inserts
• Genomic DNA cloning

Pack Size:

Price:

Inquiry

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Datasheet

Applications

Features:

• Streptomycin resistant
• High transformation efficiency

Applications:

• Suitable for genomic DNA cloning
• Allow large inserts
• Cloning of methylated cytosine or adenine containing DNA
• Blue/white screening

Quality Control:

Store At:

- 70℃

ECOS™ 10B (DH10B)

Product No.

FYE508-80VL

ECOS X.png

Specification & Description:

DH10B is an MC1061 derivative. This strain was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids.
While DH10B has been classically reported to be galU galK, the preliminary genome sequence for DH10B indicates that DH10B is actually galE galK galU+. Genome sequence indicates that DH10B is actually deoR+. Complete genome sequence has been published.

> 2 x 108, 100 μl x 80 vials

> 2 x 108 cfu/μg
Recommended for:
• Cloning of large inserts
• Genomic DNA cloning

Pack Size:

Price:

Inquiry

Get a Quote
arrow&v
Submit Request

Datasheet

Applications

Features:

• Streptomycin resistant
• High transformation efficiency

Applications:

• Suitable for genomic DNA cloning
• Allow large inserts
• Cloning of methylated cytosine or adenine containing DNA
• Blue/white screening

Quality Control:

Store At:

- 70℃

ECOS™ 2163 (GM2163)

Product No.

FYE807-10VL

ECOS X.png

Specification & Description:

100 μl x 10 vials

> 5 x 107, 100 μl x 10 vials

Pack Size:

Price:

Inquiry

Get a Quote
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Submit Request

Datasheet

Applications

Features:

Applications:

Quality Control:

Store At:

- 70℃

ECOS™ 2163 (GM2163)

Product No.

FYE807-80VL

ECOS X.png

Specification & Description:

100 μl x 80 vials

> 5 x 107, 100 μl x 80 vials

Pack Size:

Price:

Inquiry

Get a Quote
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Submit Request

Datasheet

Applications

Features:

Applications:

Quality Control:

Store At:

- 70℃

ECOS™ Sonic (Acella™)

Product No.

HYE007-Sonic-5VL

ECOS X.png

Specification & Description:

100 μl x 5 vials

> 5 x 108, 100 μl x 5 vials

Pack Size:

Price:

Inquiry

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